Gram-negativeBacilliZoonotic

Pasteurella multocida

Gram-negative coccobacillus predominantly transmitted through animal bites and scratches, causing rapidly progressive soft-tissue infections, and less commonly pneumonia, septicemia, or meningitis.

Organism Card

DomainMust know
Identity
  • G−ve coccobacillus (short rod); non-motile, non-sporing
  • Facultative anaerobe; belongs to family Pasteurellaceae
  • Bipolar staining ("safety-pin" appearance) on Gram stain
Lab discriminator
  • Oxidase +, catalase +, indole + (distinguishes from many other Pasteurellaceae)
  • Grows on blood agar & chocolate agar; does NOT grow on MacConkey agar (key discriminator vs Enterobacteriaceae)
  • No hemolysis on blood agar; musty/mousy odor on culture
  • vs Bartonella henselae (cat-scratch disease): Bartonella requires prolonged incubation, oxidase −; Pasteurella grows rapidly (24–48 h)
Reservoir / transmission
  • Normal oral flora of cats (70–90%) and dogs (50–60%)
  • Transmission: cat/dog bite or scratch; cat bites higher risk than dog bites
  • Licking of open wounds also sufficient
  • HK-relevant: high pet density; consider in any bite wound presenting to A&E
Key virulence
  • Polysaccharide capsule → antiphagocytic, promotes invasive disease
  • LPS (endotoxin) → sepsis in immunocompromised
  • Rapid tissue invasion → explains very short incubation ( < 24 h, often < 12 h after bite)
Clinical syndromes
  • Cellulitis/wound infection within 24 hours of cat or dog bite — hallmark presentation
  • Rapidly progressive: intense erythema, swelling, seropurulent discharge at bite site
  • Complications: tenosynovitis, septic arthritis, osteomyelitis (especially hand bites)
  • Disseminated disease in immunocompromised/asplenic: bacteraemia, meningitis, peritonitis, pneumonia
  • Respiratory tract infections in patients with chronic lung disease (COPD colonisation)
Diagnosis
  • Specimen: wound swab / aspirate from bite site; blood cultures if systemic signs
  • Gram stain: G−ve coccobacilli; culture on blood/chocolate agar (no MacConkey growth)
  • Rapid onset ( < 24 h) after animal bite is the key clinical clue that narrows differential
  • Pitfall: may be dismissed as "contaminant" if history of animal contact not elicited
Treatment
  • First-line: amoxicillin-clavulanate (co-amoxiclav) — covers Pasteurella + oral anaerobes in bite wounds
  • Alternatives (penicillin-allergic): doxycycline, fluoroquinolones, TMP-SMX (variable)
  • Pasteurella is intrinsically resistant to first-generation cephalosporins (e.g. cephalexin) and macrolides (erythromycin) — classic exam trap
  • Severe/disseminated: IV penicillin G or IV ampicillin-sulbactam
  • Always assess tetanus status and need for rabies post-exposure prophylaxis in bite wounds
Prevention
  • Thorough wound irrigation and debridement immediately after bite
  • Prophylactic antibiotics recommended for: cat bites (high infection rate ~50%), deep puncture wounds, hand/face bites, immunocompromised patients
  • Primary wound closure generally avoided for bite wounds (except face for cosmesis)
  • No vaccine available
Classic traps
  • Cat bite + cellulitis < 24 h = Pasteurella (vs dog bite + cellulitis > 24 h more likely Capnocytophaga canimorsus, especially if asplenic)
  • Capnocytophaga → fulminant sepsis in asplenic; Pasteurella → local wound infection in immunocompetent
  • Bartonella henselae = cat scratch disease → regional lymphadenopathy, subacute (weeks); Pasteurella = acute wound infection (hours)
  • Erysipelothrix rhusiopathiae → fish/meat handler; Eikenella corrodens → human bite
  • Do NOT use cephalexin alone for cat bites — misses Pasteurella

Exam Intelligence

  1. GC 178 (An ugly wound): Animal bite wounds are a classic setting for Pasteurella multocida infection — the lecture emphasises wound infection pathogens and antiseptic technique; cat/dog bites are a core scenario [1].

  2. GC 105 (Medically important microbes): Pasteurella multocida is listed among medically important G−ve organisms every doctor should know — emphasised as the prototypical bite wound organism alongside Capnocytophaga [1].

  3. GC 106 (Practical issues in antibiotic use): Co-amoxiclav is the empirical antibiotic of choice for animal bite wounds — this is directly examinable as a prescribing question; the lecture warns against using cephalexin alone [1].

  4. GC 098 (Antibiotic prophylaxis): Cat bites warrant prophylactic antibiotics due to high infection rates (~50%) — the lecture frames prophylaxis indications by wound type, location, and host factors [1].

  5. AOS Microbiology material: Pasteurella is a "classic vignette" organism — very short stem required: animal bite + rapid-onset cellulitis = Pasteurella multocida [2].

  • Onset timing is the key discriminator: Pasteurella causes infection within 12–24 hours of the bite. If the vignette says infection developed "3 days later" after a dog bite, consider Staphylococcus aureus, Streptococcus, or Capnocytophaga instead.
  • Cat bite vs dog bite: Cat bites are deeper puncture wounds, higher inoculation → higher Pasteurella infection rate. Dog bites → broader polymicrobial flora, also risk of Capnocytophaga canimorsus.
  • Asplenic patient + dog bite + fulminant sepsis = Capnocytophaga canimorsus (NOT Pasteurella — this is a common wrong answer).
  • Cat scratch + regional lymphadenopathy (subacute, weeks) = Bartonella henselae (cat-scratch disease), NOT Pasteurella.
  • Human bite + "clenched fist" injury = Eikenella corrodens, NOT Pasteurella.
  • First-generation cephalosporin resistance: A favourite wrong-answer trap — students may choose cephalexin for "skin infection," but Pasteurella is intrinsically resistant. Always pick co-amoxiclav.

No directly relevant past paper questions concerning Pasteurella multocida were identified in the indexed Fourth Summative papers (2016–2025 MCQ, SAQ, and Minicase papers). The organism has not appeared as a named answer or stem pathogen in the available indexed past paper context.

However, note that the 2021 Fourth Summative SAQ Q12 [3] asks about organisms in immunocompromised settings (post-BMT), and the 2018 Fourth Summative Minicase [4] asks to name organisms causing specific infections — students should be prepared to list Pasteurella in any bite wound or soft tissue infection scenario if it appears in future papers.

[1] Lecture slides: GC 178 (An ugly wound), GC 105 (Medically important microbes), GC 106 (Practical issues in antibiotic use), GC 098 (Antibiotic prophylaxis) [2] AOS material: AOS - Microbio.pdf [3] Past papers: 2021 Fourth Summative SAQ.pdf [4] Past papers: 2018 Fourth Summative Minicase.pdf

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